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Microscopy home

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Sample prep lab

 

JEOL 2010F

JEOL 1200 EX

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JEOL 6330F

Hitachi 4700 F

Hitachi 3000

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RHK VT SPM100

JEOL 5200 SPM

DI/Veeco 3100 SPM

 

PHI XPS

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Olympus Vanox DIC

Olympus Vanox Fluor.

JEOL 6330F SEM

scanning electron microscope

 

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Electron beam energy: 0.2-30 keV, 

Resolution: 1.5 nm, 

Magnification: 500,000x, 

3D imaging package, 

  

Energy dispersive spectrometer (EDS), X-Ray range: 0.15-30 keV Energy resolution.

Microscope:              JEOL 6330F

​Service contractor:  EM Control

Manager:                  Carroll​

Location: â€‹                  RM07 microscopy 

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Resources

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Documentation

 

SEM Instructions​

Book on SEM

Getting Started

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To get started with the JEOL JSM-6330F Scanning Electron Microscope (SEM), you'll need to understand the basics of sample insertion, operating the SEM software, and adjusting settings for optimal image viewing. 

 

Sample Insertion:

Open the chamber door: Gently open the chamber door, which is a manual process.

Turn and pull the knob: Locate the knob on the chamber door and turn it towards you (a quarter turn). Pull the knob out to release the door.

Insert the sample: Carefully push your sample stage onto the dovetail mount to secure it in place within the SEM chamber. 

 

Operating the SEM Software and Adjusting Settings:

Ensure accelerating voltage is off:

Before making any adjustments, verify that the accelerating voltage is turned off. 

 

Select desired voltage:

Choose the appropriate accelerating voltage you need for your sample and imaging requirements. 

 

Engage accelerating voltage:

Once selected, push the accelerating voltage button to activate it. 

 

Wait for emission current:

The emission current should stabilize at the desired level (e.g., 12 microamps). 

 

Adjust brightness and contrast:

Use the brightness and contrast controls to optimize the image for clear viewing. 

 

Avoid security screen:

Never press Ctrl, Alt, and Delete simultaneously while the SEM control software is running, as this can cause the SEM image to disappear and the PC to freeze. 

 

Mouse pointer and animation settings:

Be aware that certain mouse settings (e.g., "Make Mouse Pointer Shadow Effective" or "Animate Menus and Hints") may affect the SEM image display, potentially causing disturbances. 

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If you encounter specific issues or need more detailed guidance:

Consult the JSM-6330 manual

Seek further assistance

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Wake Forest University is a small, selective, research university located in Winston-Salem NC. It has  a long tradition of innovation in the biomedical and physical sciences.  NanoteQ is a unit of WFU serving as a university-wide  central facility for specialized equipment and capabilities. Images and data content of this website are the property of WFU and its affiliate colleges. NanoteQ @ Wake reserves editorial right of access to commentary on these pages. Opinions expressed are not those of the University and the site does not represent binding policy by the University.

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